She made her foray into the world of pornographic movies during her junior year, in 1984. Her debut performance was in the film Educating Nina which was produced by the veteran porn star Juliet Anderson (better known as Aunt Peg), and this proved to be a massive hit. Since then she has gone on to feature in over 400 first-run adult films, and has become one of the most enduring and recognizable performers in the industry.

She is best known within the adult film industry for her "bubble butt" (often referred to as the "best ass in the business"), interracial scenes and her lesbian scenes.

Nina has won numerous awards throughout her career, from various critic and fan organizations. The following is a list of her Adult Video News Awards.

# Cheating Housewives 4 (2007) (V)
# It's a Mommy Thing! (2007) (V)
# Momma Knows Best 3 (2007) (V)
# My Friend's Hot Mom 8 (2007) (V)
# Shorty Iz Fuckin' Yo Mama (2007) (V)
# White Wife Black Cock 8 (2007) (V)
# Bondage Babes (2006) (V)
# Group Sex 5 (2006) (V)
# Guide to Erotic Massage (2006) (V)
# Guide to Foot Fun (2006) (V)
# Guide to Porn Stars Sex Secrets (2006) (V)
... aka Nina Hartley's Guide to Porn Stars Sex Secrets (USA: complete title)